Scophthalmus maximus cystatin B enhances head kidney macrophage-mediated bacterial killing

doi:10.1016/j.dci.2010.07.008

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	Scophthalmus maximus cystatin B enhances head kidney macrophage-mediated bacterial killing
	Xiao, Ping-ping 1,2; Hu, Yong-hua 1; Sun, Li 1
	2010-12-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	34 期号:12 页码:1237-1241
文章类型	Article
摘要	Cystatins form a large family of cysteine protease inhibitors found in a wide arrange of organisms. Studies have indicated that mammalian cystatins play important roles under both physiological and pathological conditions. However, much less is known about fish cystatins. In this report, we described the identification and analysis of a cystatin B homologue, SmCytB, from turbot Scophthalmus maximus. The open reading frame of SmCytB is 300 bp, which encodes a 99-residue protein that shares high levels of sequence identities with the cystatin B of a number of fish species and contains the conserved cysteine protease inhibitor motif of cystatin B. Constitutive expression of SmCytB is high in muscle, brain, heart and liver, and low in spleen. blood, gill and kidney. Bacterial infection upregulates SmCytB expression in kidney, spleen, liver and brain but not in muscle or heart. Functional analysis showed that recombinant SmCytB purified from Escherichia colt exhibits apparent cysteine protease inhibitor activity. Transient overexpression of SmCytB in head kidney macrophages enhances macrophage bactericidal activity probably through a nitric oxide-independent mechanism. These results indicate that SmCytB is involved in the immune defense of turbot against bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved.; Cystatins form a large family of cysteine protease inhibitors found in a wide arrange of organisms. Studies have indicated that mammalian cystatins play important roles under both physiological and pathological conditions. However, much less is known about fish cystatins. In this report, we described the identification and analysis of a cystatin B homologue, SmCytB, from turbot Scophthalmus maximus. The open reading frame of SmCytB is 300 bp, which encodes a 99-residue protein that shares high levels of sequence identities with the cystatin B of a number of fish species and contains the conserved cysteine protease inhibitor motif of cystatin B. Constitutive expression of SmCytB is high in muscle, brain, heart and liver, and low in spleen. blood, gill and kidney. Bacterial infection upregulates SmCytB expression in kidney, spleen, liver and brain but not in muscle or heart. Functional analysis showed that recombinant SmCytB purified from Escherichia colt exhibits apparent cysteine protease inhibitor activity. Transient overexpression of SmCytB in head kidney macrophages enhances macrophage bactericidal activity probably through a nitric oxide-independent mechanism. These results indicate that SmCytB is involved in the immune defense of turbot against bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved.
关键词	Cystatin b Scophthalmus Maximus Macrophage Bacterial Infection
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2010.07.008
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000283270700001
引用统计	被引频次：34[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5914
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Xiao, Ping-ping,Hu, Yong-hua,Sun, Li. Scophthalmus maximus cystatin B enhances head kidney macrophage-mediated bacterial killing[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2010,34(12):1237-1241.
APA	Xiao, Ping-ping,Hu, Yong-hua,&Sun, Li.(2010).Scophthalmus maximus cystatin B enhances head kidney macrophage-mediated bacterial killing.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,34(12),1237-1241.
MLA	Xiao, Ping-ping,et al."Scophthalmus maximus cystatin B enhances head kidney macrophage-mediated bacterial killing".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 34.12(2010):1237-1241.

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