Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis | |
Li, Chenghua1; Qiu, Limei2; Ning, Xuanxuan3; Chen, Aiqin1; Qin, Song1; Wu, Huifeng1; Zhao, Jianmin1 | |
2010-09-01 | |
发表期刊 | FISH & SHELLFISH IMMUNOLOGY |
ISSN | 1050-4648 |
卷号 | 29期号:3页码:530-533 |
文章类型 | Article |
摘要 | Translationally controlled tumor protein (TCTP) is one of the abundant and ubiquitously expressed proteins in metazoans In the present study, the first molluscan TCTP (denoted as VpTCTP) was identified from Venerupis philippinarum haemocytes by EST and RACE approaches The full-length cDNA of VpTCTP consisted of 1148 nucleotides with an open-reading frame of 555 bp encoding 184 amino acids The deduced amino acid sequence of VpTCTP shared high similarity with TCTPs from other species, indicating that VpTCTP should be a new member of TCTP family Several highly conserved motifs, including 5'terminal ologopyrimidine (5'TOP) starting sequence and rich AU and AUUT elements in 3'UTR, were also identified in VpTCTP The tissue and temporal expression of VpTCTP after Vi boo anguillarum challenge was recorded by quantitative real-time RT-PCR. VpTCTP transcript could be detected in all examined tissues with the highest expression level in haemocytes and the lowest in hepatopancreas Concerning the time-course expression in haemocytes, the relative expression of VpTCTP mRNA was down-regulated sharply from 6 h to 12 h post-infection. Then, the expression level was obviously up-regulated and reached 3.4-fold to that in the control group at 48 h post challenge As time progressed, the expression of VpTCTP recovered to the original level at 96 h. All these results indicated that VpTCTP was an acute-phase protein involved in the Immune response of V philippinarum (C) 2010 Elsevier Ltd. All rights reserved.; Translationally controlled tumor protein (TCTP) is one of the abundant and ubiquitously expressed proteins in metazoans In the present study, the first molluscan TCTP (denoted as VpTCTP) was identified from Venerupis philippinarum haemocytes by EST and RACE approaches The full-length cDNA of VpTCTP consisted of 1148 nucleotides with an open-reading frame of 555 bp encoding 184 amino acids The deduced amino acid sequence of VpTCTP shared high similarity with TCTPs from other species, indicating that VpTCTP should be a new member of TCTP family Several highly conserved motifs, including 5'terminal ologopyrimidine (5'TOP) starting sequence and rich AU and AUUT elements in 3'UTR, were also identified in VpTCTP The tissue and temporal expression of VpTCTP after Vi boo anguillarum challenge was recorded by quantitative real-time RT-PCR. VpTCTP transcript could be detected in all examined tissues with the highest expression level in haemocytes and the lowest in hepatopancreas Concerning the time-course expression in haemocytes, the relative expression of VpTCTP mRNA was down-regulated sharply from 6 h to 12 h post-infection. Then, the expression level was obviously up-regulated and reached 3.4-fold to that in the control group at 48 h post challenge As time progressed, the expression of VpTCTP recovered to the original level at 96 h. All these results indicated that VpTCTP was an acute-phase protein involved in the Immune response of V philippinarum (C) 2010 Elsevier Ltd. All rights reserved. |
关键词 | Venerupis Philippinarum Tctp Tissues Expression Time-course Expression |
学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
DOI | 10.1016/j.fsi.2010.04.018 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000280513700020 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/5942 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Chenghua,Qiu, Limei,Ning, Xuanxuan,et al. The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis[J]. FISH & SHELLFISH IMMUNOLOGY,2010,29(3):530-533. |
APA | Li, Chenghua.,Qiu, Limei.,Ning, Xuanxuan.,Chen, Aiqin.,Qin, Song.,...&Zhao, Jianmin.(2010).The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis.FISH & SHELLFISH IMMUNOLOGY,29(3),530-533. |
MLA | Li, Chenghua,et al."The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis".FISH & SHELLFISH IMMUNOLOGY 29.3(2010):530-533. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
The first molluscan (451KB) | 限制开放 | -- | 浏览 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论