Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Expression of beta-carotene hydroxylase gene (crtR-B) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii | |
Tan, Cong-Ping; Zhao, Fang-Qing; Su, Zhong-Liang; Liang, Cheng-Wei; Qin, Song | |
2007-08-01 | |
发表期刊 | JOURNAL OF APPLIED PHYCOLOGY |
ISSN | 0921-8971 |
卷号 | 19期号:4页码:347-355 |
文章类型 | Article |
摘要 | A carotenoid gene (crtR-B) from the green alga Haematococcus pluvialis, encoding beta-carotene hydroxylase that was able to catalyze the conversion of beta-carotene to zeaxanthin and canthaxanthin to astaxanthin, was cloned into Chlamydomonas reinhardtii chloroplast expression vector p64D to yield plasmid p64DcrtR-B. The vector p64DcrtR-B was transferred to the chloroplast genome of C. reinhardtii using micro-particle bombardment. PCR and Southern blot analyses indicated that crtR-B was integrated into the chloroplast genome of the transformants. RTPCR assays showed that the H. pluvialis crt R-B gene was expressed in C. reinhardtii transformants. The transformants rapidly synthesized carotenoids in larger quantities than the wild-type upon being transferred from moderate to high-intensity white light. This research provides a foundation for further study to elucidate the possible mechanism of photo-protection by xanthophylls and other carotenoids in high light conditions or through exposure to UV radiation.; A carotenoid gene (crtR-B) from the green alga Haematococcus pluvialis, encoding beta-carotene hydroxylase that was able to catalyze the conversion of beta-carotene to zeaxanthin and canthaxanthin to astaxanthin, was cloned into Chlamydomonas reinhardtii chloroplast expression vector p64D to yield plasmid p64DcrtR-B. The vector p64DcrtR-B was transferred to the chloroplast genome of C. reinhardtii using micro-particle bombardment. PCR and Southern blot analyses indicated that crtR-B was integrated into the chloroplast genome of the transformants. RTPCR assays showed that the H. pluvialis crt R-B gene was expressed in C. reinhardtii transformants. The transformants rapidly synthesized carotenoids in larger quantities than the wild-type upon being transferred from moderate to high-intensity white light. This research provides a foundation for further study to elucidate the possible mechanism of photo-protection by xanthophylls and other carotenoids in high light conditions or through exposure to UV radiation. |
关键词 | Chlamydomonas Reinhardtii Chloroplast Transformation Beta-carotene Hydroxylase Gene (Crtr-b) Carotenoids |
学科领域 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
DOI | 10.1007/s10811-006-9141-8 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000248188000006 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/5994 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Shandong Inst Light Ind, Coll Food & Bioengn, Jinan 250100, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Ocean Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China |
推荐引用方式 GB/T 7714 | Tan, Cong-Ping,Zhao, Fang-Qing,Su, Zhong-Liang,et al. Expression of beta-carotene hydroxylase gene (crtR-B) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii[J]. JOURNAL OF APPLIED PHYCOLOGY,2007,19(4):347-355. |
APA | Tan, Cong-Ping,Zhao, Fang-Qing,Su, Zhong-Liang,Liang, Cheng-Wei,&Qin, Song.(2007).Expression of beta-carotene hydroxylase gene (crtR-B) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii.JOURNAL OF APPLIED PHYCOLOGY,19(4),347-355. |
MLA | Tan, Cong-Ping,et al."Expression of beta-carotene hydroxylase gene (crtR-B) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii".JOURNAL OF APPLIED PHYCOLOGY 19.4(2007):347-355. |
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