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PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus)
Liu Huaide1,2; Liu Mei1; Wang Baojie1,2; Jiang Keyong1; Jiang Shan1,2; Sun Shujuan1,2; Wang Lei1
2010-07-01
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
卷号28期号:4页码:808-814
文章类型Article
摘要In this study, the intestinal microbiota of kuruma shrimp (Marsupenaeus japonicus) was examined by molecular analysis of the 16S rDNA to identify the dominant intestinal bacteria and to investigate the effects of Bacillus spp. on intestinal microbial diversity. Samples of the intestines of kuruma shrimp fed normal feed and Bacillus spp. amended feed. PCR and denaturing gradient gel electrophoresis (DGGE) analyses were then performed on DNA extracted directly from the guts. Population fingerprints of the predominant organisms were generated by DGGE analysis of the universal V3 16S rDNA amplicons, and distinct bands in the gels were sequenced. The results suggested that the gut of kuruma shrimp was dominated by Vibrio sp. and uncultured gamma proteobacterium. Overall, the results of this study suggest that PCR-DGGE is a possible method of studying the intestinal microbial diversity of shrimp.; In this study, the intestinal microbiota of kuruma shrimp (Marsupenaeus japonicus) was examined by molecular analysis of the 16S rDNA to identify the dominant intestinal bacteria and to investigate the effects of Bacillus spp. on intestinal microbial diversity. Samples of the intestines of kuruma shrimp fed normal feed and Bacillus spp. amended feed. PCR and denaturing gradient gel electrophoresis (DGGE) analyses were then performed on DNA extracted directly from the guts. Population fingerprints of the predominant organisms were generated by DGGE analysis of the universal V3 16S rDNA amplicons, and distinct bands in the gels were sequenced. The results suggested that the gut of kuruma shrimp was dominated by Vibrio sp. and uncultured gamma proteobacterium. Overall, the results of this study suggest that PCR-DGGE is a possible method of studying the intestinal microbial diversity of shrimp.
关键词Intestinal Microbial Diversity Pcr-dgge Kuruma Shrimp
学科领域Limnology ; Oceanography
DOI10.1007/s00343-010-9101-7
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收录类别SCI
语种英语
WOS记录号WOS:000279294400014
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被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/6154
专题海洋生物技术研发中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
第一作者单位中国科学院海洋研究所
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GB/T 7714
Liu Huaide,Liu Mei,Wang Baojie,et al. PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus)[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(4):808-814.
APA Liu Huaide.,Liu Mei.,Wang Baojie.,Jiang Keyong.,Jiang Shan.,...&Wang Lei.(2010).PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus).CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(4),808-814.
MLA Liu Huaide,et al."PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus)".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.4(2010):808-814.
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