IOCAS-IR  > 海洋生物技术研发中心
Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR
Wang, Haiyan1,2; Guo, Ximing1
2008-05-01
发表期刊JOURNAL OF SHELLFISH RESEARCH
ISSN0730-8000
卷号27期号:3页码:481-487
文章类型Article
摘要Genetic markers are needed for rapid and reliable identification of oysters. In this study, we developed multiplex genus- and species-specific PCR markers for the identification of oysters from China. We used the mitochondrial cytochrome oxidase I (COI) and nuclear 28S ribosomal RNA genes for marker development. DNA sequences from different species were obtained from GenBank or by direct sequencing. Sequences were aligned, and genus- and species-specific nucleotides were identified. Primers were designed for genus/species-specific amplification to generate fragments of different sizes. A multiplex set of genus- and species-specific primers from the 28S gene was able to separate C. ariakensis and C. hongkongensis from other species and assign oysters to four genera. A set of species-specific COI primers provided positive identification of all five Crassostrea species from China, C. ariakensis, C. hongkongensis, C. angulata, C. gigas, and C. sikamea in a single PCR. The multiplex PCR assays do not require fluorescence-labeling or post-PCR enzyme digestion, providing a simple, fast and reliable method for the identification of oysters from China.; Genetic markers are needed for rapid and reliable identification of oysters. In this study, we developed multiplex genus- and species-specific PCR markers for the identification of oysters from China. We used the mitochondrial cytochrome oxidase I (COI) and nuclear 28S ribosomal RNA genes for marker development. DNA sequences from different species were obtained from GenBank or by direct sequencing. Sequences were aligned, and genus- and species-specific nucleotides were identified. Primers were designed for genus/species-specific amplification to generate fragments of different sizes. A multiplex set of genus- and species-specific primers from the 28S gene was able to separate C. ariakensis and C. hongkongensis from other species and assign oysters to four genera. A set of species-specific COI primers provided positive identification of all five Crassostrea species from China, C. ariakensis, C. hongkongensis, C. angulata, C. gigas, and C. sikamea in a single PCR. The multiplex PCR assays do not require fluorescence-labeling or post-PCR enzyme digestion, providing a simple, fast and reliable method for the identification of oysters from China.
关键词Oyster Identification Species-specific Pcr Cytochrome Oxidase i 28s Rrna Ostreidae Suminoe Oyster Crassostrea Ariakensis
收录类别SCI
语种英语
WOS记录号WOS:000256117200001
引用统计
被引频次:78[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/6330
专题海洋生物技术研发中心
作者单位1.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Piscataway, NJ 08349 USA
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
第一作者单位中国科学院海洋研究所
推荐引用方式
GB/T 7714
Wang, Haiyan,Guo, Ximing. Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR[J]. JOURNAL OF SHELLFISH RESEARCH,2008,27(3):481-487.
APA Wang, Haiyan,&Guo, Ximing.(2008).Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR.JOURNAL OF SHELLFISH RESEARCH,27(3),481-487.
MLA Wang, Haiyan,et al."Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR".JOURNAL OF SHELLFISH RESEARCH 27.3(2008):481-487.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
wang-Identification (401KB) 限制开放--浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Haiyan]的文章
[Guo, Ximing]的文章
百度学术
百度学术中相似的文章
[Wang, Haiyan]的文章
[Guo, Ximing]的文章
必应学术
必应学术中相似的文章
[Wang, Haiyan]的文章
[Guo, Ximing]的文章
相关权益政策
暂无数据
收藏/分享
文件名: wang-Identification of Crassostrea ariakensis and related oysters by multiplex.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。