| Cloning and characterization of a novel caspase-8-like gene in Crassostrea gigas | |
Li, Chunyan1,2 ; Qu, Tao1; Huang, Baoyu1,2; Ji, Peng1,2; Huang, Wen1,2; Que, Huayong1; Li, Li1; Zhang, Guofan1
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| 2015-10-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| 卷号 | 46期号:2页码:486-492 |
| 文章类型 | Article |
| 摘要 | Cysteine-dependent aspartate-directed proteases, or caspases, play key roles in apoptosis and immune defense. In this study, we cloned the first caspase-8-like gene (CgCaspase8-2) identified in the pacific oyster, Crassostrea gigas. The 2572-bp cDNA encodes a putative protein of 714 amino acids that contains two tandem death effector domains (DEDs) at the N-terminal, and P20 and P10 domains at the C-terminal. The conserved pentapeptide motif QACQG was also identified in the deduced CgCaspase8-2 protein. Phylogenetic analysis indicated that CgCaspase8-2 was clustered with initiator caspases in the invertebrate subgroup, but the similarity between CgCaspase8-2 and other invertebrate caspase-8s was low. CgCaspase8-2 protein was localized in the cytoplasm, and over-expression of CgCaspase8-2 in HEK293T cells induced cell death, suggesting a role in apoptosis. Quantitative real-time PCR results demonstrated that CgCaspase8-2 was widely expressed in various tissues and developmental stages, with the highest CgCaspase8-2 expression levels detected in hemolymph and the blastula stage. Furthermore, CgCaspase8-2 transcripts showed no change in response to a bacterial challenge but exhibited notable up-regulation post-poly (I:C) challenge, suggesting that CgCctspase8-2 is specifically involved in immune responses against viruses. In summary, CgCaspase8-2 is involved in both apoptotic and immune function. (C) 2015 Elsevier Ltd. All rights reserved. |
| 关键词 | Crassostrea Gigas Cgcaspase8-2 Apoptosis Immune Response |
| DOI | 10.1016/j.fsi.2015.06.035 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000363071200037 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/73936 |
| 专题 | 海洋生物技术研发中心 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Chunyan,Qu, Tao,Huang, Baoyu,et al. Cloning and characterization of a novel caspase-8-like gene in Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2015,46(2):486-492. |
| APA | Li, Chunyan.,Qu, Tao.,Huang, Baoyu.,Ji, Peng.,Huang, Wen.,...&Zhang, Guofan.(2015).Cloning and characterization of a novel caspase-8-like gene in Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,46(2),486-492. |
| MLA | Li, Chunyan,et al."Cloning and characterization of a novel caspase-8-like gene in Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 46.2(2015):486-492. |
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| Cloning and characte（848KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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