Direct immobilisation of antibodies on a bioinspired architecture as a sensing platform

doi:10.1016/j.bios.2010.11.013

	Direct immobilisation of antibodies on a bioinspired architecture as a sensing platform
	Wan, Yi 1,2; Zhang, Dun 1; Wang, Yi1 ; Qi, Peng1,2 ; Hou, Baorong 1; Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
	2011-01-15
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
卷号	26 期号:5 页码:2595-2600
文章类型	Article
摘要	A sensitive and selective immunosensor for the nonlabeled detection of sulfate-reducing bacteria (SRB) is constructed using a self-polymerised polydopamine film as the immobilisation platform. Self-polymerisation of dopamine is used as a powerful approach for applying multifunctional coatings onto the surface of a gold electrode. The polydopamine film is used not only as the immobilisation platform, but also as a cross-linker reagent for the immobilisation of the anti-SRB antibody. The polydopamine film is loaded with a high density of anti-SRB antibodies linked to the substrate to obtain high response signals. The formation and fabrication of the biosensor and the quantification of antibody anchoring are monitored, and SRB detection is performed by either quartz crystal microbalance (QCM) or electrochemical impedance spectroscopy (EIS). After modeling the impedance Nyquist plots of the SRB/anti-SRB/polydopamine/gold electrode for increasing concentrations of SRB, the electron transfer resistance (R(ct)) is used as a measure of immunocomplex binding. The R(ct) is correlated with the concentration of bacterial cells in the range of 1.8 x 10(2) to 1.8 x 10(6) CFU mL(-1); the detection limit is 50 CFU mL(-1). This work demonstrates a new immobilisation platform for the development of a sensitive and label-less impedimetric and piezoelectric immunosensor. This immunosensor may be broadly applied in clinical diagnoses and the monitoring of water environmental pollution. The method proposed is distinct in its ease of application, use of a simple protocol, and mild reaction conditions. These allow it to be applied to a wide variety of materials. (C) 2010 Elsevier B.V. All rights reserved.; A sensitive and selective immunosensor for the nonlabeled detection of sulfate-reducing bacteria (SRB) is constructed using a self-polymerised polydopamine film as the immobilisation platform. Self-polymerisation of dopamine is used as a powerful approach for applying multifunctional coatings onto the surface of a gold electrode. The polydopamine film is used not only as the immobilisation platform, but also as a cross-linker reagent for the immobilisation of the anti-SRB antibody. The polydopamine film is loaded with a high density of anti-SRB antibodies linked to the substrate to obtain high response signals. The formation and fabrication of the biosensor and the quantification of antibody anchoring are monitored, and SRB detection is performed by either quartz crystal microbalance (QCM) or electrochemical impedance spectroscopy (EIS). After modeling the impedance Nyquist plots of the SRB/anti-SRB/polydopamine/gold electrode for increasing concentrations of SRB, the electron transfer resistance (R(ct)) is used as a measure of immunocomplex binding. The R(ct) is correlated with the concentration of bacterial cells in the range of 1.8 x 10(2) to 1.8 x 10(6) CFU mL(-1); the detection limit is 50 CFU mL(-1). This work demonstrates a new immobilisation platform for the development of a sensitive and label-less impedimetric and piezoelectric immunosensor. This immunosensor may be broadly applied in clinical diagnoses and the monitoring of water environmental pollution. The method proposed is distinct in its ease of application, use of a simple protocol, and mild reaction conditions. These allow it to be applied to a wide variety of materials. (C) 2010 Elsevier B.V. All rights reserved.
关键词	Dopamine Impedimetric Immunosensor Sensing Platform Bioinspired Architecture Bacteria Detection
学科领域	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
DOI	10.1016/j.bios.2010.11.013
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000286904400130
引用统计	被引频次：57[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11437
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wan, Yi,Zhang, Dun,Wang, Yi,et al. Direct immobilisation of antibodies on a bioinspired architecture as a sensing platform[J]. BIOSENSORS & BIOELECTRONICS,2011,26(5):2595-2600.
APA	Wan, Yi,Zhang, Dun,Wang, Yi,Qi, Peng,Hou, Baorong,&Zhang, D .(2011).Direct immobilisation of antibodies on a bioinspired architecture as a sensing platform.BIOSENSORS & BIOELECTRONICS,26(5),2595-2600.
MLA	Wan, Yi,et al."Direct immobilisation of antibodies on a bioinspired architecture as a sensing platform".BIOSENSORS & BIOELECTRONICS 26.5(2011):2595-2600.

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