Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad | |
Wu, Ning1,2; Yu, Ai-Bing3; Zhu, Hua-Bin2; Lin, Xiu-Kun1,2; Lin, XK (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. | |
2012 | |
发表期刊 | JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY |
ISSN | 1110-7243 |
页码 | 343891 |
文章类型 | Article |
摘要 | Delivering siRNA or shRNA into the developing embryos is still a main challenge to use of RNAi in mammalian systems. Here we analyze several factors influencing RNAi-mediated silencing of Sry gene, which is a tightly controlled spatiotemporal expressed gene and only shortly expressed in developing mouse embryo gonad. A Sry gene-specific shRNAs expression vector (pSilencer4.1/Sry565) was constructed. The shRNA constructs were mixed with polyethylenimines (PEIs) to form a complex and then injected into pregnant mice though tail vein. Our results showed that Sry gene was downregulated significantly in developing embryos. Further study revealed that knocking-down of Sry expression resulted in feminization of gonad development in mouse embryos and the expression level of Sox9 and Wt1 gene was also significantly changed by downregulation of Sry. The transfection efficiency is associated with the amount of plasmid DNA injection, injection time, injection speed, and volume. Our studies suggest that transplacental RNAi could be implemented by tail vein injection of plasmid vector into pregnant mice. |
关键词 | Sex-determining Gene Pre-sertoli Cells Testis Development In-vivo Mammalian-cells Plasmid Dna Expression Mice Wt1 Sox9 |
学科领域 | Biotechnology & Applied Microbiology ; Medicine, Research & Experimental |
DOI | 10.1155/2012/343891 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000301372700001 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/12317 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Lin, XK (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100094, Peoples R China 3.Yangzhou Univ, Anim Nutr Lab, Grad Sch Anim Sci & Technol, Yangzhou 225009, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Wu, Ning,Yu, Ai-Bing,Zhu, Hua-Bin,et al. Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad[J]. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY,2012:343891. |
APA | Wu, Ning,Yu, Ai-Bing,Zhu, Hua-Bin,Lin, Xiu-Kun,&Lin, XK .(2012).Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad.JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY,343891. |
MLA | Wu, Ning,et al."Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad".JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY (2012):343891. |
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