Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Comparative study of four flagellins of Vibrio anguillarum: Vaccine potential and adjuvanticity | |
Jia, Pan-pan1,2; Hu, Yong-hua2; Chi, Heng2; Sun, Bo-guang2; Yu, Wen-gong1; Sun, Li2; Sun, L | |
2013-02-01 | |
发表期刊 | FISH & SHELLFISH IMMUNOLOGY |
ISSN | 1050-4648 |
卷号 | 34期号:2页码:514-520 |
文章类型 | Article |
摘要 | Vibrio anguillarum is the etiological agent of vibriosis, an aquaculture disease that affects a wide range of farmed fish. The genome of V anguillarum contains five flagellin genes, i.e. flaA,flaB,flaC,flaD, and flaE. In this study, we analyzed the vaccine potential and adjuvanticity of FlaA, FlaB, FlaD, and FlaE in a model of Japanese flounder (Paralichthys olivaceus). For this purpose, recombinant FlaA, FlaB, FlaD, and FlaE were expressed in and purified from Escherichia coli. In vivo immunogenicity analysis showed that antibodies against rFlaA, rFlaB, rFlaD, and rFlaE were detected in rat antiserum raised against live V. anguillarum, with the highest antibody level being that against rFlaB. When administered into flounder via intraperitoneal injection, rFlaA, rFlaD, and rFlaE induced comparable relative percent survival (RPS) rates, which were significantly lower than that induced by rFlaB. Specific serum antibodies were induced by all flagellins, however, the antibody level induced by rFlaB was significantly higher than those induced by other three flagellins. Compared to sera from fish vaccinated with rFlaA, rFlaD, and rFlaE, serum from fish vaccinated with rFlaB significantly reduced the infectivity of V. anguillarum against host cells. To examine the potential adjuvant effect of the flagellins, flounder were immunized with rEsa1, a D15-like surface antigen that induces protective immunity as a subunit vaccine, in the presence or absence of rFlaA, rFlaB, rFlaD, and rFlaE respectively. The results showed that rFlaE, but not other three flagellins, significantly increased the RPS of rEsa1. Compared to fish vaccinated with rEsa1, fish vaccinated with rEsa1 plus rFlaE exhibited a significantly higher level of serum antibodies and enhanced expression of the genes involved in innate and adaptive immunity. Taken together, these results indicate that FlaA, FlaB, FlaD, and FlaE have different immunological properties and, as a result, differ in vaccine and adjuvant potentials. (C) 2012 Elsevier Ltd. All rights reserved.; Vibrio anguillarum is the etiological agent of vibriosis, an aquaculture disease that affects a wide range of farmed fish. The genome of V anguillarum contains five flagellin genes, i.e. flaA,flaB,flaC,flaD, and flaE. In this study, we analyzed the vaccine potential and adjuvanticity of FlaA, FlaB, FlaD, and FlaE in a model of Japanese flounder (Paralichthys olivaceus). For this purpose, recombinant FlaA, FlaB, FlaD, and FlaE were expressed in and purified from Escherichia coli. In vivo immunogenicity analysis showed that antibodies against rFlaA, rFlaB, rFlaD, and rFlaE were detected in rat antiserum raised against live V. anguillarum, with the highest antibody level being that against rFlaB. When administered into flounder via intraperitoneal injection, rFlaA, rFlaD, and rFlaE induced comparable relative percent survival (RPS) rates, which were significantly lower than that induced by rFlaB. Specific serum antibodies were induced by all flagellins, however, the antibody level induced by rFlaB was significantly higher than those induced by other three flagellins. Compared to sera from fish vaccinated with rFlaA, rFlaD, and rFlaE, serum from fish vaccinated with rFlaB significantly reduced the infectivity of V. anguillarum against host cells. To examine the potential adjuvant effect of the flagellins, flounder were immunized with rEsa1, a D15-like surface antigen that induces protective immunity as a subunit vaccine, in the presence or absence of rFlaA, rFlaB, rFlaD, and rFlaE respectively. The results showed that rFlaE, but not other three flagellins, significantly increased the RPS of rEsa1. Compared to fish vaccinated with rEsa1, fish vaccinated with rEsa1 plus rFlaE exhibited a significantly higher level of serum antibodies and enhanced expression of the genes involved in innate and adaptive immunity. Taken together, these results indicate that FlaA, FlaB, FlaD, and FlaE have different immunological properties and, as a result, differ in vaccine and adjuvant potentials. (C) 2012 Elsevier Ltd. All rights reserved. |
关键词 | Vibrio Anguillarum Flagellin Vaccine Adjuvant |
学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
DOI | 10.1016/j.fsi.2012.11.039 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
WOS记录号 | WOS:000315076100012 |
WOS关键词 | MUCOSAL IMMUNE-RESPONSES ; TOLL-LIKE RECEPTOR-5 ; FISH CELL-LINE ; BACTERIAL FLAGELLIN ; PARALICHTHYS-OLIVACEUS ; INNATE IMMUNITY ; EXPRESSION ; MOTILITY ; PROTEIN ; SALMONELLA |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/16547 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Sun, L |
作者单位 | 1.Ocean Univ China, Sch Med & Pharm, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
第一作者单位 | 实验海洋生物学重点实验室 |
推荐引用方式 GB/T 7714 | Jia, Pan-pan,Hu, Yong-hua,Chi, Heng,et al. Comparative study of four flagellins of Vibrio anguillarum: Vaccine potential and adjuvanticity[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(2):514-520. |
APA | Jia, Pan-pan.,Hu, Yong-hua.,Chi, Heng.,Sun, Bo-guang.,Yu, Wen-gong.,...&Sun, L.(2013).Comparative study of four flagellins of Vibrio anguillarum: Vaccine potential and adjuvanticity.FISH & SHELLFISH IMMUNOLOGY,34(2),514-520. |
MLA | Jia, Pan-pan,et al."Comparative study of four flagellins of Vibrio anguillarum: Vaccine potential and adjuvanticity".FISH & SHELLFISH IMMUNOLOGY 34.2(2013):514-520. |
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