Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
DNA porn-porn nanostructure as a multifunctional platform for pathogenic bacteria determination and inactivation | |
Zeng, Yan1,2,3; Qi, Peng1,2,3; Wang, Yingwen1,2,3,4; Chen, Chao1,2,3; Zhang, Dun1,2,3 | |
2021-04-01 | |
发表期刊 | BIOSENSORS & BIOELECTRONICS |
ISSN | 0956-5663 |
卷号 | 177页码:9 |
通讯作者 | Qi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn) |
摘要 | Pathogenic bacteria levels are significantly related with disease control, clinical diagnosis, and even environmental monitoring. It is becoming highly urgent to achieve ultrasensitive detection of pathogenic bacteria and efficient combat of bacterial infection. Toward this end, we have assembled a DNA Pom-Pom nanostructure (PP-N) based multifunctional platform for pathogenic bacteria determination and inactivation. In particular, one DNA oligonucleotide probe that serve as a trigger was specifically designed for the autonomous cross-opening of metastable DNA hairpin probes and long dsDNA structure formation, achieving a catalytic self-assembly of DNA nanostructure. Numerous DNA strands in this PP-N assembly provide sufficient interaction sites for functional domains and connector, showing high programmability, excellent biostability, as well as selective target recognition. With these properties, the fluorescence dyes modified PP-N platform showed excellent bacteria analysis with both excellent selectivity and ultrasensitive determination limit as low as 2.0 CFU/mL. Furthermore, the aptamer-functionalized and antibiotics loaded PP-N platform demonstrate excellent merits of high antibiotics-loading capacity and negligible cytotoxicity to targets. Therefore, this DNA PP-N assembly based multifunctional platform promise its great application in targeted sensing, combating bacterial infection, and potential clinic therapy. |
关键词 | DNA nanostructure Pathogenic bacteria Ultrasensitive detection Bacteria inactivation Antibiotics loading |
DOI | 10.1016/j.bios.2021.112982 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[41906037] ; National Natural Science Foundation of China[41876101] ; Young Elite Scientists Sponsorship Program by CAST[20180276] |
WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
WOS记录号 | WOS:000618826100001 |
出版者 | ELSEVIER ADVANCED TECHNOLOGY |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/169607 |
专题 | 海洋环境腐蚀与生物污损重点实验室 |
通讯作者 | Qi, Peng; Zhang, Dun |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
第一作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
通讯作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
推荐引用方式 GB/T 7714 | Zeng, Yan,Qi, Peng,Wang, Yingwen,et al. DNA porn-porn nanostructure as a multifunctional platform for pathogenic bacteria determination and inactivation[J]. BIOSENSORS & BIOELECTRONICS,2021,177:9. |
APA | Zeng, Yan,Qi, Peng,Wang, Yingwen,Chen, Chao,&Zhang, Dun.(2021).DNA porn-porn nanostructure as a multifunctional platform for pathogenic bacteria determination and inactivation.BIOSENSORS & BIOELECTRONICS,177,9. |
MLA | Zeng, Yan,et al."DNA porn-porn nanostructure as a multifunctional platform for pathogenic bacteria determination and inactivation".BIOSENSORS & BIOELECTRONICS 177(2021):9. |
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文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
1-s2.0-S095656632100(8640KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 |
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