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Simultaneous ultrasensitive ADP and ATP quantification based on CRISPR/ Cas12a integrated ZIF-90@Ag3AuS2@Fe3O4 nanocomposites | |
Wang, Yingwen1,2,3; Zhang, Dun1,2,3; Zeng, Yan1,3; Sun, Yan1,3; Qi, Peng1,2,3 | |
2022-12-15 | |
发表期刊 | BIOSENSORS & BIOELECTRONICS |
ISSN | 0956-5663 |
卷号 | 218页码:10 |
通讯作者 | Zhang, Dun(zhangdun@qdio.ac.cn) ; Qi, Peng(qipeng@qdio.ac.cn) |
摘要 | ADP/ATP ratio is a sensitive indicator of changes in cellular energy status and is important for regulating cell signaling activities. Ultrasensitive quantification of ADP and ATP concentration in a single system is in great demand for bioanalysis and early disease diagnosis. Hence, a target-regulated luminous nanoplatform based on clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a integrated zeolite imidazolate framework (ZIF-90)@Ag3AuS2@Fe3O4 nanocomposites was established for the simultaneous detection of ADP and ATP. This simultaneous and ultrasensitive quantification nanoplatform (dsDNA-ZIF-90@Ag3AuS2@Fe3O4) composed an ADP sensitive module based on the aptamer-activated CRISPR/Cas12a and an ATP responsive module based on ATP-triggered ZIF-90 decomposition and quencher loading release. The binding and signaling processes of the different nucleotides were independent, and there was no interference between the two modules. Utilizing the high specific binding and strong signal amplification of this method, limits of detection as low as 0.022 and 0.079 nM were obtained within 30 min for ADP and ATP, respectively. Moreover, the proposed biosensor exhibited high accuracy, specificity, and excellent recovery in serum samples and bacterial biofilms. Therefore, the dsDNA-ZIF-90@Ag3AuS2@Fe3O4-based nanoplatform provides a promising method for ultrasensitive dual-mode quantification of ADP and ATP in the same system, possessing great potential for bioanalysis and early disease diagnosis. |
关键词 | Simultaneous detection Ultrasensitive quantification CRISPR Cas12a ZIF-90@Ag3AuS2@Fe3O4 ADP and ATP |
DOI | 10.1016/j.bios.2022.114784 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China ; Nantong Scientific Plan Foundation ; Open fund of Wenzhou Key Laboratory of Sanitary Microbiology ; Shandong Provincial Marine Science and Technology Projects ; [41876101and 41906037] ; [JC2021054] ; [JC2020090] ; [ZD202003KF01] |
WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
WOS记录号 | WOS:000876432600005 |
出版者 | ELSEVIER ADVANCED TECHNOLOGY |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/180504 |
专题 | 海洋环境腐蚀与生物污损重点实验室 |
通讯作者 | Zhang, Dun; Qi, Peng |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Wang, Yingwen,Zhang, Dun,Zeng, Yan,et al. Simultaneous ultrasensitive ADP and ATP quantification based on CRISPR/ Cas12a integrated ZIF-90@Ag3AuS2@Fe3O4 nanocomposites[J]. BIOSENSORS & BIOELECTRONICS,2022,218:10. |
APA | Wang, Yingwen,Zhang, Dun,Zeng, Yan,Sun, Yan,&Qi, Peng.(2022).Simultaneous ultrasensitive ADP and ATP quantification based on CRISPR/ Cas12a integrated ZIF-90@Ag3AuS2@Fe3O4 nanocomposites.BIOSENSORS & BIOELECTRONICS,218,10. |
MLA | Wang, Yingwen,et al."Simultaneous ultrasensitive ADP and ATP quantification based on CRISPR/ Cas12a integrated ZIF-90@Ag3AuS2@Fe3O4 nanocomposites".BIOSENSORS & BIOELECTRONICS 218(2022):10. |
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