Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Knockout of a diatom cryptochrome by CRISPR/Cas9 causes an increase in light-harvesting protein levels and accumulation of fucoxanthin | |
Yang, Wenting1,2,3; Zhou, Lu2,3,4; Wang, Jing2; Wang, Lijun2,3; Gao, Shan2,3; Wang, Guangce2,3 | |
2022-07-01 | |
发表期刊 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS |
ISSN | 2211-9264 |
卷号 | 66页码:9 |
通讯作者 | Gao, Shan(shangao@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn) |
摘要 | Diatoms are a promising source of fucoxanthin, a carotenoid that has many beneficial effects in human health. However, the gene encoding the last step of the fucoxanthin biosynthesis pathway in diatoms remains uniden-tified, and the molecular regulatory mechanisms are unknown. In this study, we show that cryptochrome CryP, a blue light-sensitive protein in the diatom Phaeodactylum tricornutum regulates fucoxanthin biosynthesis. CryP transcripts showed a diurnal rhythmic expression pattern; however, CryP protein showed high stability under different photoperiods. Furthermore, CryP not only regulates the expression of genes encoding carotenoid biosynthesis, but also affects the level of fucoxanthin chlorophyll a/c-binding proteins (FCPs). Upon knockout of the CryP gene, no change in cell morphology or growth was observed. However, both fucoxanthin content and FCP levels in P. tricornutum knockout lines increased greatly compared with the wild type. Our study is the first to report cryptochrome knockout in diatoms, which opens the possibility of fucoxanthin production through genome editing in P. tricornutum targeting known crucial steps. |
关键词 | Fucoxanthin Cryptochrome Phaeodactylum tricornutum |
DOI | 10.1016/j.algal.2022.102822 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Key Research and Development Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41976097] ; National Natural Science Foundation of China[41876163] ; Major Scientific and Technological Innovation Projects in Shandong Province[2019JZZY010815] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science ; Chinese Academy of Sciences[COMS2019Q02] ; China Agriculture Research System of MOF and MARA[CARS-50] |
WOS研究方向 | Biotechnology & Applied Microbiology |
WOS类目 | Biotechnology & Applied Microbiology |
WOS记录号 | WOS:000861083100005 |
出版者 | ELSEVIER |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/180718 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Gao, Shan; Wang, Guangce |
作者单位 | 1.Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Yang, Wenting,Zhou, Lu,Wang, Jing,et al. Knockout of a diatom cryptochrome by CRISPR/Cas9 causes an increase in light-harvesting protein levels and accumulation of fucoxanthin[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,66:9. |
APA | Yang, Wenting,Zhou, Lu,Wang, Jing,Wang, Lijun,Gao, Shan,&Wang, Guangce.(2022).Knockout of a diatom cryptochrome by CRISPR/Cas9 causes an increase in light-harvesting protein levels and accumulation of fucoxanthin.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,66,9. |
MLA | Yang, Wenting,et al."Knockout of a diatom cryptochrome by CRISPR/Cas9 causes an increase in light-harvesting protein levels and accumulation of fucoxanthin".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 66(2022):9. |
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