Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
The integrative expression of GUS gene driven by FCP promoter in the seaweed Laminaria japonica (Phaeophyta) | |
Li, Fuchao; Qin, Song; Jiang, Peng; Wu, Yun; Zhang, Wei | |
2009-06-01 | |
发表期刊 | JOURNAL OF APPLIED PHYCOLOGY |
ISSN | 0921-8971 |
卷号 | 21期号:3页码:287-293 |
文章类型 | Article |
摘要 | In this study, the background activity of beta-glucuronidase (GUS) was analyzed histochemically and fluorometrically in the negative control of Laminaria japonica (Phaeophyta) thalli, showing low level of activity. GUS gene transformation without selectable gene in L. japonica was performed using four different promoters, i.e., Cauliflower mosaic virus 35S promoter (CaMV35S) from cauliflower mosaic virus, ubiquitin promoter (UBI) from maize, adenine-methyl transfer enzyme gene promoter (AMT) from virus in green alga Chlorella, and fucoxanthin chlorophyll a/c-binding protein gene promoter (FCP) from diatom Phaeodactylum tricornutum. The GUS transient activity was determined fluorometrically after bombarding sliced parthenogenetic sporophytes explants, and it was found that the activity resulting from CaMV35S and FCP promoters (in 114.3 and 80.6 pmol MU min(-1) (mg protein)(-1), respectively) was higher than for the other two promoters. The female gametophytes were bombarded and regenerated parthenogenetic sporophytes. FCP was the only promoter that resulted in detectable GUS chimeric expression activity during histochemical staining and polymerase chain reaction. Results of Southern blot showed that GUS gene was integrated with the L. japonica genome.; In this study, the background activity of beta-glucuronidase (GUS) was analyzed histochemically and fluorometrically in the negative control of Laminaria japonica (Phaeophyta) thalli, showing low level of activity. GUS gene transformation without selectable gene in L. japonica was performed using four different promoters, i.e., Cauliflower mosaic virus 35S promoter (CaMV35S) from cauliflower mosaic virus, ubiquitin promoter (UBI) from maize, adenine-methyl transfer enzyme gene promoter (AMT) from virus in green alga Chlorella, and fucoxanthin chlorophyll a/c-binding protein gene promoter (FCP) from diatom Phaeodactylum tricornutum. The GUS transient activity was determined fluorometrically after bombarding sliced parthenogenetic sporophytes explants, and it was found that the activity resulting from CaMV35S and FCP promoters (in 114.3 and 80.6 pmol MU min(-1) (mg protein)(-1), respectively) was higher than for the other two promoters. The female gametophytes were bombarded and regenerated parthenogenetic sporophytes. FCP was the only promoter that resulted in detectable GUS chimeric expression activity during histochemical staining and polymerase chain reaction. Results of Southern blot showed that GUS gene was integrated with the L. japonica genome. |
关键词 | Fcp Gus Integrative Expression Laminaria Japonica Promoter |
学科领域 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
DOI | 10.1007/s10811-008-9366-9 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000265498600004 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/2964 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Li, Fuchao,Qin, Song,Jiang, Peng,et al. The integrative expression of GUS gene driven by FCP promoter in the seaweed Laminaria japonica (Phaeophyta)[J]. JOURNAL OF APPLIED PHYCOLOGY,2009,21(3):287-293. |
APA | Li, Fuchao,Qin, Song,Jiang, Peng,Wu, Yun,&Zhang, Wei.(2009).The integrative expression of GUS gene driven by FCP promoter in the seaweed Laminaria japonica (Phaeophyta).JOURNAL OF APPLIED PHYCOLOGY,21(3),287-293. |
MLA | Li, Fuchao,et al."The integrative expression of GUS gene driven by FCP promoter in the seaweed Laminaria japonica (Phaeophyta)".JOURNAL OF APPLIED PHYCOLOGY 21.3(2009):287-293. |
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