The integrative expression of GUS gene driven by FCP promoter in the seaweed Laminaria japonica (Phaeophyta)

doi:10.1007/s10811-008-9366-9

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	The integrative expression of GUS gene driven by FCP promoter in the seaweed Laminaria japonica (Phaeophyta)
	Li, Fuchao; Qin, Song; Jiang, Peng; Wu, Yun; Zhang, Wei
	2009-06-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	21 期号:3 页码:287-293
文章类型	Article
摘要	In this study, the background activity of beta-glucuronidase (GUS) was analyzed histochemically and fluorometrically in the negative control of Laminaria japonica (Phaeophyta) thalli, showing low level of activity. GUS gene transformation without selectable gene in L. japonica was performed using four different promoters, i.e., Cauliflower mosaic virus 35S promoter (CaMV35S) from cauliflower mosaic virus, ubiquitin promoter (UBI) from maize, adenine-methyl transfer enzyme gene promoter (AMT) from virus in green alga Chlorella, and fucoxanthin chlorophyll a/c-binding protein gene promoter (FCP) from diatom Phaeodactylum tricornutum. The GUS transient activity was determined fluorometrically after bombarding sliced parthenogenetic sporophytes explants, and it was found that the activity resulting from CaMV35S and FCP promoters (in 114.3 and 80.6 pmol MU min(-1) (mg protein)(-1), respectively) was higher than for the other two promoters. The female gametophytes were bombarded and regenerated parthenogenetic sporophytes. FCP was the only promoter that resulted in detectable GUS chimeric expression activity during histochemical staining and polymerase chain reaction. Results of Southern blot showed that GUS gene was integrated with the L. japonica genome.; In this study, the background activity of beta-glucuronidase (GUS) was analyzed histochemically and fluorometrically in the negative control of Laminaria japonica (Phaeophyta) thalli, showing low level of activity. GUS gene transformation without selectable gene in L. japonica was performed using four different promoters, i.e., Cauliflower mosaic virus 35S promoter (CaMV35S) from cauliflower mosaic virus, ubiquitin promoter (UBI) from maize, adenine-methyl transfer enzyme gene promoter (AMT) from virus in green alga Chlorella, and fucoxanthin chlorophyll a/c-binding protein gene promoter (FCP) from diatom Phaeodactylum tricornutum. The GUS transient activity was determined fluorometrically after bombarding sliced parthenogenetic sporophytes explants, and it was found that the activity resulting from CaMV35S and FCP promoters (in 114.3 and 80.6 pmol MU min(-1) (mg protein)(-1), respectively) was higher than for the other two promoters. The female gametophytes were bombarded and regenerated parthenogenetic sporophytes. FCP was the only promoter that resulted in detectable GUS chimeric expression activity during histochemical staining and polymerase chain reaction. Results of Southern blot showed that GUS gene was integrated with the L. japonica genome.
关键词	Fcp Gus Integrative Expression Laminaria Japonica Promoter
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI	10.1007/s10811-008-9366-9
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000265498600004
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2964
专题	实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Fuchao,Qin, Song,Jiang, Peng,et al. The integrative expression of GUS gene driven by FCP promoter in the seaweed Laminaria japonica (Phaeophyta)[J]. JOURNAL OF APPLIED PHYCOLOGY,2009,21(3):287-293.
APA	Li, Fuchao,Qin, Song,Jiang, Peng,Wu, Yun,&Zhang, Wei.(2009).The integrative expression of GUS gene driven by FCP promoter in the seaweed Laminaria japonica (Phaeophyta).JOURNAL OF APPLIED PHYCOLOGY,21(3),287-293.
MLA	Li, Fuchao,et al."The integrative expression of GUS gene driven by FCP promoter in the seaweed Laminaria japonica (Phaeophyta)".JOURNAL OF APPLIED PHYCOLOGY 21.3(2009):287-293.

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