Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
The expression of AmphiTCTP, a TCTP orthologous gene in amphioxus related to the development of notochord and somites | |
Chen, Zhongke; Zhang, Hongwei; Yang, Hui; Huang, Xiangwei; Zhang, Xiaohui; Zhang, Peijun | |
2007-07-01 | |
发表期刊 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY |
ISSN | 1096-4959 |
卷号 | 147期号:3页码:460-465 |
文章类型 | Article |
摘要 | The translationally controlled tumor protein (TCTP) is highly conserved and has been widely found in eukaryotic organisms. Here, we report the phylogenetic analysis and developmental expression of AmphiTCTP, a TCTP homologous gene in cephalochordate amphioxus. Phylogenetic analysis indicates that the putative protein of AmphiTCTP is close to its vertebrate orthologs. The mRNA of AmphiTCTP is found in fertilized eggs, early cleavage embryo and most of the early developmental stages by in situ hybridization and RT-PCR, but its expression is not detectable from late cleavage stage to mid-gastrula. The expression of AmphiTCTP in zygotes and early cleavage stages shows that AmphiTCTP may be a maternal gene. From the early neurula stage onward, AmphiTCTP transcript is localized in the presumptive notochord, presomitic mesoderm, and nascent somites. However, its expression is gradually down-regulated after the notochord and somites have been formed. The expression pattern of AmphiTCTP thus coincides with the differentiation of the notochord and somites, this suggests that AmphiTCTP may not be a housekeeping gene and may play an important role in mesoderm development. (c) 2007 Elsevier Inc. All rights reserved.; The translationally controlled tumor protein (TCTP) is highly conserved and has been widely found in eukaryotic organisms. Here, we report the phylogenetic analysis and developmental expression of AmphiTCTP, a TCTP homologous gene in cephalochordate amphioxus. Phylogenetic analysis indicates that the putative protein of AmphiTCTP is close to its vertebrate orthologs. The mRNA of AmphiTCTP is found in fertilized eggs, early cleavage embryo and most of the early developmental stages by in situ hybridization and RT-PCR, but its expression is not detectable from late cleavage stage to mid-gastrula. The expression of AmphiTCTP in zygotes and early cleavage stages shows that AmphiTCTP may be a maternal gene. From the early neurula stage onward, AmphiTCTP transcript is localized in the presumptive notochord, presomitic mesoderm, and nascent somites. However, its expression is gradually down-regulated after the notochord and somites have been formed. The expression pattern of AmphiTCTP thus coincides with the differentiation of the notochord and somites, this suggests that AmphiTCTP may not be a housekeeping gene and may play an important role in mesoderm development. (c) 2007 Elsevier Inc. All rights reserved. |
关键词 | Amphioxus Development Notochord Somitogenesis Amphitctp |
学科领域 | Biochemistry & Molecular Biology ; Zoology |
DOI | 10.1016/j.cbpb.2007.02.012 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000247555900013 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/5522 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | 1.Shandong Univ, Life Sci Coll, Key Lab Expt Teratol Minist Educ, Jinan 250012, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Zhongke,Zhang, Hongwei,Yang, Hui,et al. The expression of AmphiTCTP, a TCTP orthologous gene in amphioxus related to the development of notochord and somites[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2007,147(3):460-465. |
APA | Chen, Zhongke,Zhang, Hongwei,Yang, Hui,Huang, Xiangwei,Zhang, Xiaohui,&Zhang, Peijun.(2007).The expression of AmphiTCTP, a TCTP orthologous gene in amphioxus related to the development of notochord and somites.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,147(3),460-465. |
MLA | Chen, Zhongke,et al."The expression of AmphiTCTP, a TCTP orthologous gene in amphioxus related to the development of notochord and somites".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 147.3(2007):460-465. |
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